MetVBadBugs

The innate resistance of Gram-negative bacteria to antibiotics is a consequence of the combinatorial effects of two permeability barriers: the outer and inner bacterial cell membranes, their ability to efflux antibiotics out of the cell and their capacity to form antibiotic tolerant biofilms. The objectives of this project are to advance the measurement capability by providing the urgently needed essential metrology to quantitatively measure and image the localisation of antibiotics and to understand the penetration and efflux processes in bacteria and biofilms.